Effects of Different Paclobutrazol-Cultar Concentrations on the Micropropagation of Sugarcane (Saccharum Officinarum) Variety CPCL99-4455

  • Dion Daniels University of Belize, Belmopan, Belize
  • Nidia Panti University of Belize, Belmopan, Belize
  • David Guerra University of Belize, Belmopan, Belize
  • Stephen Williams University of Belize, Belmopan, Belize
Keywords: Paclobutrazol (PBZ), Cultar, Sugarcane, Micropropagation

Abstract

Sugarcane (Saccharum officinarum), being an important cash crop in Belize, accounts for 60% of agricultural exports providing employment for thousands of Belizeans. This research was carried out to determine the effects of different Paclobutrazol (PBZ-Cultar) concentrations used in the culture media on in vitro multiplication of sugarcane variety CPCL99-4455. Three PBZ-Cultar concentrations were tested and compared with the control. The parameters evaluated to determine the effects of PBZ-Cultar were average height, number of dead leaves and multiplication coefficient. The plants from this experiment were planted in the acclimatization phase to determine if the use of PBZ-Cultar had any effect of the growth and development of the plants ex vitro. The results revealed that the culture medium supplemented with 0.08% PBZ-Cultar concentration had the best results both in the multiplication phase as well as the acclimatization phase.

Author Biographies

Dion Daniels, University of Belize, Belmopan, Belize

Faculty of Science & Technology, University of Belize, Hummingbird Avenue, Belmopan, Cayo District, Belize

Nidia Panti, University of Belize, Belmopan, Belize

Faculty of Science & Technology, University of Belize, Hummingbird Avenue, Belmopan, Cayo District, Belize

David Guerra, University of Belize, Belmopan, Belize

Faculty of Science & Technology, University of Belize, Hummingbird Avenue, Belmopan, Cayo District, Belize

Stephen Williams, University of Belize, Belmopan, Belize

Faculty of Science & Technology, University of Belize, Hummingbird Avenue, Belmopan, Cayo District, Belize

References

Ali A., Naz S., Siddiqui F. and Iqbal J. (2008). An efficient protocol for large scale production of sugarcane through micropropagation. Pak. J. Bot. 40 (1): 139-149, 2008

Berova M. and Zlatev Z. (2000). Plant Growth Regulation 30: 117. https://doi.org/10.1023/A:1006300326975

Biradar S., Biradar D. P., Patil V. C., Patil S. S. and Kambar N. S. (2009). In vitro plant regeneration using shoot tip culture in commercial cultivar of sugarcane. Department of Agronomy University of Agricultural Sciences. Retrieved from: http://14.139.155.167/test5/index.php/kjas/article/viewFile/1363/1349.

Kyte L. and Kleyn J. (1996). Plants from Test Tubes: An Introduction to Micropropagation. Timber Press Inc. Print.

Latimer J. G., and Scoggins H. (2012). Using Plant Growth Regulators on Containerized Herbaceous Perennials. Virginia Cooperative extension. Retrieved from: http://pubs.ext.vt.edu/430/430-103/430-103_pdf.pdf

Murashige T. Y. and Skoog F. (1962). A revised medium for the rapid growth and bioassays with tobacco tissue culture. Physiol Plant. 15(3): 473-497.

Rahman M. N., Shaharuddin N., Wahab N., Wahab P. E., Abdullah M., Abdullah N. A., Parveez G. K., Roberts J. A. and Ramli Z. (2016). Impact of paclobutrazol on the growth and development of nursery grown clonal oil palm (Elaeis guineensis Jacq.)

Rangel-Estrada S. E., Hernández-Meneses E and Hernández-Arenas M. (2016). Micropropagación de variedades de caña de azúcar cultivadas en México. Rev. fitotec. mex vol.39 no.3 Chapingo jul./sep. 2016

Reyes Esquirol C., Jiménez Vázquez M., Bernal Villegas Jorge A., Montes de Oca Suárez L., García Fardales J. R. (2014) Enraizamiento “in vitro” y posterior aclimatación del cultivar de caña de azúcar C95-414 con el bioestimulante cubano Fitomas–E. Centro Agrícola, 41(4):87-91.

SIRDI (2015). Sugar Industry Research and Development Institute. The Sugar Industry Management Information System (SIMIS). Retrieved from agreport.bz: http://agreport.bz/2015/05/simis/

Taylor R. D. and Koo W. W. (2012). Outlook of the U.S. and World Sugar Markets. Agribusiness & Applied Economics Report 692. Retrieved from: http://ageconsearch.umn.edu/bitstream/128037/2/AAE692.pdf

Valdes T. D., Ruvalcaba L. P., Tafoya F. A., Orona C. A. L. and de Jesus Velazquez Alcaraz T. (2017) Dose of Paclobutrazol in the Growth of Sugarcane Seedlings in Vitro in the Acclimatization Stage. Agricultural Sciences, 8, 751-758.

Published
2018-08-30
How to Cite
Daniels, D., Panti, N., Guerra, D., & Williams, S. (2018). Effects of Different Paclobutrazol-Cultar Concentrations on the Micropropagation of Sugarcane (Saccharum Officinarum) Variety CPCL99-4455. JOURNAL OF ADVANCES IN BIOTECHNOLOGY, 7, 1011-1018. https://doi.org/10.24297/jbt.v7i1.7567