Differential Pulse Polarographic Behavior and Quantification of the Flucloxacillin in Pure and Pharmaceutical Dosage Forms Using a Static Mercury Drop Electrode
Differential pulse polarographic analysis (DPPA) by using static mercury drop electrode (SMDE) for quantification of flucloxacillin (FLUX) in pure and pharmaceutical dosage forms was studied. The optimum conditions for the polarographic signal were determined and a study was made of the different parameters affecting the electrochemical process. The best definition of the analytical signals was found in Britton–Robinson buffer (0.06 M) at pH 4.0 . Under the optimum conditions, liner calibration graph, Ip=f(CFLUX) was obtained in the concentration ranges of 0.1 mM (0.0494 ?g.mL-1) to 26 mM (12.8414 ?g.mL-1) at -940 to -1000 mV (versus Ag/AgCl) with relative standard deviations (RSD) did not exceed 2.4% for the concentrations of FLUX (0.0494 ?g.mL-1). Regression analysis showed a good correlation coefficient (R2=9998) between Ip and concentration over the mentioned range. The limit of detection (LOD) and the limit of quantification (LOQ) were to be 0.0040 and 0.0120 ?g.mL-1, respectively. The proposed method was validated for linearity, precision and accuracy, repeatability, sensitivity (LOD and LOQ), robustness and specificity. The developed method is applicable for the determination of FLUX in pure and different dosage forms in presence a same amount of amoxicillin (AMOX) with average recovery of 99.4 to 102.2 % and the results are in good agreement with those obtained by the HPLC reference method.
O' Neil, M. J., 2013. The Merck Index – An Encyclopedia of Chemicals, Drugs and Biologicals, Royal Society of Chemistry.
British pharmacopoeia. 2012. The Department of Health. Vol I. London: The Stationary office.
Ritchie, S.R., Rupali, P., Roberts, S.A., Thomas, M.G., 2007. Flucloxacillin treatment of staphylococcus aureus meningitis. Eur J Clin Microbiol Infect Dis. 26: 501–504.
Squella, J.A., Silva, M.M., Nunez-Vergara, L. J., 1981. Anodic polarographic determination of flucloxacillin. Talanta. 28: 855-856
Amr, L.S., Mohmed, A., Elmosallamya, Hamada M., Killaa, Mohmed M., 2013. Selective potentiometric method for determination of flucloxacillin antibiotic. J Taibah Uni Sci. 7: 195–201.
Liu, H., Wang, H., Sunderl, V.B., 2005. An isocratic ion exchange HPLC method for the simultaneous determination of flucloxacillin and amoxicillin in a pharmaceutical formulation for injection. J Pharma Bio Anal. 37(2): 395–398.
Shanmugasundaram, P., Kamal, R.R., Mohanrangan, J., Devdass, G., Arunadevi, M., Maheswari, R., Vijey Aanandhi, M., 2009. Simultaneous estimation of amoxicillin and flucloxacillin in its combined capsule dosage form by HPLC. Rasayan J chem. 2: 57-60.
Zhou, Q., Ruan, Z., Yuan, H., Juan, B., Xu, D., 2007. RP-HPLC analysis of flucloxacillin in human plasma validation and application to a bioequivalence study. Pharmazie. 62: 101–104.
Roder, B.L., Frimodt-Moller, N., Espersen, E., Rasmussen, S.N., 1995. Dicloxacillin and flucloxacillin: pharmacokinetics, protein binding and serum bactericidal titers in healthy subjects after oral administration. Infection 23: 107-112.
Zhang, M., Moore, G.A., Everts, R., Begg, E.J., 2014. Determination of Total and Free Concentrations of Flucloxacillin and Cefazolin in Human Plasma by Liquid Chromatography/Tandem Mass Spectrometry. J Anal Bioanal Tech 5(1): 2-9.
Huang C, Gao J, Miao L (2012) Simultaneous determination of flucloxacillin and ampicillin in human plasma by ultra-performance liquid chromatography tandem mass spectrometry and subsequent application to a clinical study in healthy Chinese volunteers. J Pharm Biomed Anal 59: 157-161.
Worlako Klu, M., Selorm Addy, B., Mintah D.N., 2016. A Simple Validated Rp- HPLC method for the analysis of flucloxacillin sodium in capsule dosage form, World J Pharm Pharma Sci. 5(4): 499-508.
Dey, S.A., Ratnakar, C.H., Vaithiyanathan, S., Samal, H.B., Reddy, Y.V., Krishna, Y.B., Reddy, A., Kumar, G.N., Mohapatra, S., 2010. Spectrophotometric method developed for the estimation of flucloxacilin in bulk and dosage form using UV-Vis spectrophotometric method, Int J Pharma and Bio Sci. 1(4): 35-42.
Singh, G.R., Manirul, H.S., Shanker, P., 2009. A Sensitive validated spectrophotometric method for the determination of flucloxacillin sodium, E-J of Chem. 6(S1): 397-405.
El-Mammli, M.Y., 2003. Spectrophotometric determination of flucloxacillin in pharmaceutical preparations using some nitrophenols as a complexing agent, Spec Acta Part A: Mole and Bio Spec. 59(4): 771–776.
Vijayalakshmi, R., Sowjanya, D., Archana, S., Dhanaraju, M.D., 2014. Application of extractive spectrophotometric methods for the determination of flucloxacillin and trandolapril using bromocresol green, Asian J Pharma and Clin Res. 7(4): 216-218.
Everett, J. R., Jennings, K., and Woodnutt, G. 1985. 19F NMR spectroscopy study of the metabolites of flucloxacillin in rat urine. J. Pharm. Pharmacol. 37: 869-873.
Anderson, J.E., Bond, A. M., Jones, R. D., 1981. Differential Pulse Polarography at the Static Mercury Drop Electrode, Anal. Chem. 53 (7): 1016–1020.
ICH: Proceedings of the International Conference on Harmonization of Technical Requirement of Registration of Pharmaceuticals for Human Use (ICH Harmonized Tripartite Guidelines), 2005.
Copyright (c) 2018 JOURNAL OF ADVANCES IN CHEMISTRY
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors retain the copyright of their manuscripts, and all Open Access articles are distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.