Synthesis , Characterization and Biological activity of new derivatives of Chromen-2one

We report the organic syntheses of new derivatives from Chromen-2-one and their antibacterial activity. Compounds 4-[Acetyl-(2-oxo-2H-chromen-4-yl)-amino]-benzenesulfonyl chloride 1a, 4-[Acetyl-(2oxo-3-phenylsulfamoyl-2H-chromen-4-yl)-amino]-benzenesulfonyl chloride 2a , 2-{4-[Acetyl-(4-chlorosulfonylphenyl)-amino]-2-oxo-3-phenylsulfamoyl-2H-chromen-7-ylamino}-benzoic acid 3a. All Structures have been synthesized and characterized using melting points , IR spectra , 1 H-NMR, 13 C-NMR spectra , and elemental analyses . The purified synthesized compounds (1a,2a,3a), at contcentrations 2,3,5 mg/ml was tested for their antibacterial activity against three bacterial cultures ;Staphylococcus aureus, Escherchia coli and Bacillus cereus. The antibacterial activity of synthesized compounds are compared with antibacterial activity of standard antibiotics cephalexine and streptomycine. The compounds (1a, 2a, 3a) shows different bacteriostatic and bacteriocidal activity.

Melting point was determinated on an electrothermal apparatus (Fisher Scientific 2555) in a open capillary tube and wa not corrected.Infrared spectra were recorded in cm-1 for KBr pellts on a FT-IR Shimadzu 8400S spectrophotometer with resolution 4 cm-1 , 1 H-NMR spectra were recorded on a Bruker UNITY plus-500 'NMR 1' spectrometer using DMSO-d6 as the solvent and TMS as the internal references standard ( σ = 0,00 ppm).Chemical shifts are expressed in δ ppm.Mass spectra were taken on a LKB 9000 mass spectrometer.Element analysze was performed on a Perikin-Elmer 240 BCHN analyzer.The purity of the compounds (synthesized) was routinely checked by TLC using Merck Kieselgel-60 (F-254) and benzene,toluene,glacial acetic acid (80:10:10)as mobile phase.The spots were exposed in iodine vapour for visualization.
In a 100 ml flask were mixed 3g

Antibacterial activity
The purified synthesized compounds (1a,2a,3a,) was subjected to test in vitro its antibacterial activity against three bacterial cultures ; Staphylococcus aureus,E.Coli and B.cereus.Antibacterial activity of compounds was investigated applying the Kirby-Bayer method or disc method (d=5.5 mm max.capacity 10 μg)

IV. Conclusion
From the results the following conclusion where drawn.
The study provides the first evidence that compounds (1a, 2a, 3a,) obviously inhibit the growth of S.auerus, E.coli and B.cereus.The compounds (1a, 2a, 3a) compared with the antibacterial activity of Streptomycine in S.aureus, E.coli and B.cereus.This study provided the first evidence that these compounds 1a, 2a, 3a, showed a significant antibacterial effect against S.aureus, E.coli and B.Cereus.The chemical structures of synthesizen compounds were determined according to extensive NMR experiments and published data.
Coumarine and their derivatives have shown various biological activities.Other several coumarin derivatives have antimicrobial properties (Sanghyun; et al 1996; Mohareb et al 2007; Nofal et al 2000), with reflux and condensation we have synthesize some new coumarine derivatives and to investigate their antibacterial activity against Staphylococcus aureus, E.coli and Bacillus cereus.The antibacterial activity of synthesized compounds is compared with antibacterial activity of Cefalexine and Streptomycine.

Table - 1
: characteristics and analytical data of the complexes