The productivity of wheat cultivars under salt stress not always linked with their nitrate reductase activity in leaves

Gaber K.Abd El-Baki


To evaluate the effect of salt stress on four wheat cultivars (Bani suief 1, Bani suief 3, Seds 1 and Seds 6), seeds were cultivated in sand clay soil under normal field conditions. The plants left to grow for 21-days, then treated with different concentrations of NaCl (0.0, 50, 100,150 and 200 mM NaCl) by top irrigation. The plants left till harvest around (100-days from sowing). Fresh and dry matter, spikes weight were also determined. Photosynthetic pigments (chlorophyll a, b) and carotenoides as well as total pigments were  measured. The fresh weight of four wheat cultivars ran in order (Bani suief 1> Bani suief 3> Seds 1> Seds 6) and this almost true for dry weight production. The productivity of  four wheat cultivars at 200 mM NaCl referred as (spike weight) at harvest time ran in order [Bani suief 1> Seds 6> Seds1> Bani suief 3]. Both chl. a and chl. b were showed slight reduction in most cultivars with increasing salinity. The total pigments showed various responses with different treatments. The activity of NR was increased in both cultivars (Bani suief 3 and seds 1) however, in other two cultivars (Bani suief 1 and seds 6) the activity was decreased with increasing salinity in the soil. The activity  of the enzyme at higher salinity levels used ran in order (Seds1> Bani sueif 3> Bani suief 1> Seds6).


Dry weight;Fresh weight;Harvest index;Nitrate reductase;pigments;Wheat.

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Shirazi MU, Asif SM, Khanzada B, Khan MA, Mohammad A (2001) Growth and ion accumulation in some wheat genotypes under NaCl stress. Pak. J. Biol. Sci.,4: 388-391. [2] Neumann, P (1997) Salinity resistance and plant growth revisited. Plant, Cell Environ. 20: 1193-1198. [3] Hasegawa PM, Bressan RA, Zhu JK, Bohnert HJ (2000) Plant cellular and molecular responses to high salinity. Ann. Rev. Plant Physiol. Plant Mol. Biol. 51: 463–499. [4] Hamada, EAM, Hamond MA, El-sayed MA, Kirkwood RC, El-Sayed H (1992) Studies on adaptation of selected species of the family Gramineae. Fedds Repertorium, 103: 87-98. [5] Khan D, Shaukat SS, Faheemuddin M (1984) Germination Studies of certain plants. Pak. J. Bot., 16: 231-254. [6] Flores P, Botella MA, Martínez V, Cerdá A (2000) Ionic and osmotic effects of nitrate reductase activity in tomato seedlings. J. Plant Physiol. 156:552–557. [7] Seckin B, Sekmen AH, Turkan I (2009) An enhancing effect of exogenous mannitol on the antioxidant enzymes activities in roots of wheatb under salt stress. J. Plant growth Regu. 28:12:20. [8] Huang, L, Murray F, Yang X (1993) Responses of nitrogen metabolism parameters to sub lethal SO2 pollution in wheat [Triticum aestivum cv-Wilgoyne (Ciana/Gallo)] under mild NaCl stress. Environ. and Exp. Bot., 33: 479-493. [9] Zornoza P, Gonzales M (1998) Intraspecific differences in nitrogen assimilating enzymes in spinach under contrasting forms of nitrogen supply. J. Plant Nutr. 21: 1129-1138. [10] Carillo P, Mastrolonardo G, Nacca F, Fuggi A (2005) Nitrate reductase in durum wheat seedlings as affected by nitrate nutrition and salinity. Func. Plant Biol. 32: 209–219. [11] Botella MA, Cruz C, Martins-Loucao M, Cerda A (1993) Nitrate reductase activity in wheat seedlings as affected by NO3-/NH4+ ratio and salinity. J. Plant Physiol.142: 531-536. [12] Kenjebaeva S, Rakova N (1995) Multiple forms of nitrate reductase and their role in nitrate assimilation in roots of wheat at low temperature or high salinity. Physiol. Plant. 93: 249-252. [13] Metzner H, Rau H, Senger H. (1965) Untersuchungen zur synchronisierbarkareit einzelener-pigment. Mangel Mutanten von Chlorella. Planta 65: 186-194. [14] Szabolcs I (1994) Prospects of soil salinity for the 21st century. 15th World Congress of Soil Science. Acapulco, July 10–16 1994, I:123–141. [15] Halperin ST, Gilroy S, Lynch JP (2003) Sodium chloride reduces growth and cytosolic calcium, but does not affect cytosolic pH, in root hairs of Arabidopsis thaliana L. J. Exp. Bot. 54: 1269–1280. [16] Gunes A, Inal A, Alpaslam M, Erslan F, Bagsi EG, Cicek N (2007) Salicylic acid induced changes on some physiological parameters symptomatic for oxidative stress and mineral nutrition in maiz (Zea mays L.) grown under salinity. J. Plant physiol. 164: 728-736. [17] Zhang X., Wang J, Huang J, Lan H, Wang C, Yin C, Wu Y, Tang H, Qian Q, Lin J et al. (2012) Rare allele of OsPPKL1 associated with grain length causes extra-large grain and a significant yield increase in rice. Proc. Natl. Acad. Sci. USA 109: 21534–21539. [18] Munns R (2003) Comparative physiology of salt and water stress. Plant Cell Environ. 25(2): 239-250. [19] Lazof D, Bernstein N (1997) The NaCl-induced inhibition of shoot growth. The case for disturbed nutrition with special consideration of calcium nutrition. Adv. Bot. Res., 29: 115-189. [20] Mensah, J.K.: Akomeah, P.A.; Ikhajiagbe B.; Ekpekurede E.O. (2006): Effect of salinity on germination, growth and yield of five ground nut genotypes. African journal of Biotechnology 5(20):1973-1979. [21] Tejera NA, Sussi M., Lluch C (2006) Physiological and nutritional indicators of tolerance to salinity in Chickpea plants growing under symbiotic conditions. Environ. Exp. Bot. 58 (1-3): 17-24. [22] Murphy KM, Lyon SR, Balow KA, Jones SS (2010) Post-sexual cycle regrowth and grain yield in Thinopyrum elongatum x Triticum aestivum amphiploids. Plant Breed. 129:480–483. [23] Aslam M, Qureshi RH, Ahmed N (1993) A rapid screening techniques for salt tolerance in rice (Oryza sativa L) plant and soil 150: 99-107. [24] Zhu GY, Kinet JM, Lutts S (2001) Characterization of rice (Oryza sativa L.) F3 populations selected for salt resistance. I. Physiological behaviour during vegetative growth. Euphytica 121, 25–263. [25] Soussi M, Ocana A, Lluch C (1998) Effect of salt stress on growth, photosynthesis, and nitrogen fixation in Checkpea (Cicer arietimum L.). J. Exp. Bot., 49: (325):1329-1337. [26] Sinel’nikova VN, Kosareva IA, Bazhanov IA (1998) Effect of chloride salinity on functional changes in the photosynthetic apparatus of tomato varieties. Sbovnik Nauchnykh Trudov PoPrikladnoi Botanike., Genetike i Selektsii 116: 64–71. [27] Pandey UK, Saxena HK (1987) Effect of soil salinity on chlorophyll, photosynthesis, respiration and ionic composition at various growth stages in paddy. Indian J. Agric. Chem. 20:149–155. [28] Salma ST, Busheva SM, Arafa AA, Garab G, Erdei L (1994) Effect of NaCl salinity on growth, cation accumulation, chloroplast structure and function in wheat cultivars differing in salt tolerance. J. Plant Physiol. 144: 241–247. [29] Moharekar ST, Lokhande SD, Hara T, Tanaka R, Tanaka A, Chavan PD (2003) Effect of salicylic acid on chlorophyll and carotenoid contents of wheat and moong seedlings. Photosynthetica 41(2), 315-317. [30] Kaya C, Kirnak H, Higgs D, Saltati K (2002) Supplementary calcium enhances plant growth and fruit yield in strawberry cultivars grown at high (NaCl) salinity. Scientia Horti. 26, 807-820. [31] Parida, AK, Das AB (2005) Salt Tolerance and Salinity Effects on Plants: A Review Ecotox. Environ. Safe. 60: 324-349. [32] El-Tayeb MA (2005) Response of barley grains to the interactive effect of salinity and salicylic acid. Plant Growth Regul. 45(3), 215–224. [33] Türkyılmaz B, Aktaş LY, Güven A (2005) Salicylic acid induced some biochemical and physiological changes in Phaseolus vulgaris L. Sci. Engin. J. Firat Univ. 17(2), 319-326. [34] Yildirim E, Turan M, Guvenc I ( 2008) Effect of foliar salicylic acid applications on growth, chlorophyll and mineral content of cucumber (Cucumis sativus L.) grown under salt stress. J. Plant Nutr. 31: 593-612. [35] Apse MP, Aharon GS, Snedden WA, Blumwald E. (1999) Salt tolerance conferred by overexpression of a vacuolar Na+/H+ antiport in Arabidopsis. Science 285:1256–1258. [36] Calza R, Huttner E, Vincentz M, Rauze P, Vaucheret H, CherelI et al. (1987) Complimentary to tobacco nitrate reductase m-RNA and encoding epitopes common to the nitrate reductase from higher plants. Mol. Gen. Genet. 209: 552–562. [37] Srivastava H.S. (1995) Nitrate reductase. In: Srivastava A. and Singh R.P. (eds), Nitrogen Nutrition in Higher Plants. Associated Publishing Company, New Delhi, pp. 145–164. [38] Larcher W(1995) Physiological Plant Ecology-Ecophysiology and Stress Physiology of Functional Groups. 3rd edn. Springer, Tokyo. [39] Martnez V, Cerda A (1989b) Nitrate reductase in tomato and cucumber leaves as influenced by NaCl and N source. J. Plant Nutr. 12: 1335-1350. [40] Abd El-Baki GK, Siefrt ZF, Man HM, Weiner H, Kaldenhoff R, Kaiser WM (2000) Nitrate reductase in Zea mays L. under salinity. Plant Cell Environ. 23:515-521. [41] Misra AN, Dwiverdi UN (1990) Nitrogen assimilation in germinating Phaseolus aureus seeds under saline stress. J. plant Physiol. 135: 719-724. [42] Aslam M, Huffaker RC, Rains DW, (1984) Early effect of salinity on nitrate assimilation in barley seedlings. Plant Physiol. 76: 321-325. [43] Cramer GR, Schierholt A, Wang YZ, lips SH (1995) The influence of salinity on utilization of root anaplerotic carbon and nitrogen metabolism in tomato seedlings. J. Exp. Bot. 46: 1569-1577. [44] Kaiser WM, Huber SC (2001) Post translational regulation of nitrate reductase, mechanism, physiological relevance and environmental triggers. J. Exp. Botany 52: 1981-1989. [45] Viegas RA, Melo ARB, Silveira JAG (1999) Nitrate reductase activity and proline accumulation in cashew (Anacardium occidentale L.) in response to salt (NaCl) shock. Braz. J. Plant Physiol. 11:21–28. [46] Wang R, Tischner R, Rodrigo AG, Hoffman M, Xing X, Chen M, Coruzzi G, Crawford NM (2004) Genomic analysis of the nitrate response using a nitrate reductase-null mutant of arabidopsis. Plant Physiol. 136: 2512–2522. [47] Foyer CH, Valadier M, Migge A, Becker TW (1998) Drought–induced effects on nitrate reductase activity and m-RNA and on coordination of nitrogen and carbon in maize leaves. Plant Physiol. 117: 283–292. [48] Bybordi A, Ebrahimian E (2011) Effect of Salinity Stress on Activity of Enzymes Involved in Nitrogen and Phosphorous Metabolism Case Study: Canola (Brassica napus L.). Asian J. Agric. Sci., 5:208-214. [49] Carvajal M, Martnez V, Alcaraz FC ( 1999) Physiological function of water channels as affected by salinity in roots of paprika pepper. Physiol. Planta.105: 95–101. [50] Parida AK, Das AB (2004) Effects of NaCl stress on nitrogen and phosphorus metabolism in a true mangrove Bruguiera parviflora grown under hydroponic culture. J. Plant Physiol. 161:921–928. [51] Deane-Drummond CE ( 1986) A comparison of regulatory effects of chloride on nitrate uptake, and of nitrate on chloride uptake into Pisum sativum seedlings. Physiol. Planta. 66:115–126. [52] Lin H, Sandra SS, Schumaker KS (1997) Salt sensitivity and the activities of the H-ATPase in cotton seedlings. Crop Sci.. 37:190–197. [53] Sivasnakar S, Oaks A (1996) Nitrate assimilation in higher plants the effect of metabolism and light. Plant Physiol. Biochem. 34; 609-620. [54] Abdul-kadir SM, Paulsen GM (1982) Effect of salinity on nitrogen metabolism in wheat. J. plant Nutr. 5: 1141-1151.


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