JOURNAL OF ADVANCES IN AGRICULTURE https://cirworld.com/index.php/jaa Interested in submitting to this journal? We recommend that you review the About the Journal page for the journal's section policies, as well as the Author Guidelines. Authors need to register with the journal prior to submitting or, if already registered, can simply log in and begin the five-step process. KHALSA PUBLICATIONS en-US JOURNAL OF ADVANCES IN AGRICULTURE 2349-0837 <p>Authors retain the copyright of their manuscripts, and all Open Access articles are distributed under the terms of the&nbsp;<a href="https://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</a>, which permits unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.</p> Impact of Biochar and Different Nitrogen Sources on Forage Radish Production in Middle Tennessee https://cirworld.com/index.php/jaa/article/view/8035 <p>Short-season forage radish (<em>Raphanus sativus</em> L. var. longipinnatus) has recently gained great popularity in Middle Tennessee and many parts of the world used as a high-quality vegetable crop for human consumption or a forage crop for winter grazing and cover cropping. In this study, we (i) estimated soil pH buffering capacity and microbial activity, (ii) quantified crop productivity influenced by different biochar amendment rates and N fertilizer management practices based on a factorial treatment design. Particularly, biochar was amended at rates of 0, 5, 20, and 40 Mg/ha; N fertilizer was applied at zero (N0), 122 kg/ha of urea (56 kg/ha of N; N1) and 4.8 Mg/ha of aged dairy cattle manure (56-60 kg/ha of N), providing a total of 12 treatments (four biochar rates × three fertilization practices). The combination of biochar and inorganic N fertilizer such as urea appeared to have positive impacts on the short-term biomass production, soil pH buffering capacity, and enhanced soil microbial activity for short-season forage radish production (<em>P</em> &lt; 0.05). Future research is warranted to evaluate the use of biochar in field-based forage/vegetable studies in Tennessee.</p> Todd Pirtle Lee Rumble Michael Klug Forbes Walker Song Cui Nathan Phillips ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2019-01-31 2019-01-31 10 1594 1610 10.24297/jaa.v10i0.8035 Asexual Propagation of Four Medicinal Greek Endemic Plants of Lamiaceae Family With Conservation Priority From The Collection of The Balkan Botanic Garden of Kroussia, N. Greece https://cirworld.com/index.php/jaa/article/view/8039 <p>Conservation of endemic rare-threatened plants and sustainable exploitation of biodiversity with emphasis on medicinal-aromatic plants and plants with horticultural/ornamental value can be achieved through ex situ conservation activities. For this purpose, propagation experiments with cuttings were performed on four local Ionian endemic species with conservation priority, <em>Stachys ionica </em>Halácsy, <em>Teucrium halascyanum</em>, <em>Thymus holosericeus C</em>elak and <em>Thymus plasonii </em>Adamovic (all Lamiaceae). For propagation, softwood tip cuttings (3-5 cm) were cut at early autumn from mother plants collected from the wild and maintained in open-air mother plantations. For experimentation, the base of cuttings was immersed for 1 min in solutions of four concentrations of IBA (0, 1000, 2000 and 4000 ppm). Cuttings were placed on a peat:perlite (1:3) substrate in the bench of greenhouse heated mist system. Most suitable treatment for <em>T. halascyanum</em> (3 ½ weeks) proved to be 1000 ppm IBA (32.13 roots 1.72 cm long, 100% rooting). Accordingly, 2000 ppm IBA gave 100% rooting for both <em>S. ionica</em> (28.5 roots 1.56 cm long,) (3 weeks) and <em>Th. holosericeus</em> (4.4 roots 1.76 cm long) (7 weeks). <em>T. plasonii</em> cuttings treated with 2000 ppm IBA gave 85.71% optimum rooting with 8.67 roots 1.78 cm long.</p> Virginia Sarropoulou Eleni Maloupa ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2019-01-31 2019-01-31 10 1611 1622 10.24297/jaa.v10i0.8039 Increasing Eggs Protein Level and Eggshell Integrality Performed by Addition of Xylanase, Amylase, Protease (Avizyme® 1502) in Layers Feed https://cirworld.com/index.php/jaa/article/view/8061 <p>The high number of broken eggshells on laying hens in Indonesia is very detrimental to farmers, so that needs to be overcome. A total of 480 Lohman chickens with 40 weeks of age were divided into 4 treatment groups with the addition of different doses of Avizyme® 1502 respectively P0 as a control that got 0 g/Kg of feed, P1 got 1 g/Kg of feed, P2 got 2 g/Kg of feed, P3 got 3 g/Kg of feed. Avizyme® 1502 contains enzymes xylanase, amylase, and protease. This treatment was given daily for 30 days. The sampling of eggshell checking was recorded when start of administering enzymes to the end of the administration and at the end of the study, 6 eggs from each treatment were taken randomly to measure the protein level. The results showed that the addition of 1 g/Kg of Avizyme® 1502 on feed provided the highest protein level in eggs. During the research period, eggs produced by the groups of chicken under treatments of Avizyme 1502® showed a decrease in cracked eggshell.</p> Hamong Suharsono Ida Bagus Putu Semara Putra Ida Bagus Komang Ardana I Wayan Nico Fajar Gunawan Putu Henrywaesa Sudipa Kadek Karang Agustina ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2019-02-28 2019-02-28 10 1623 1628 10.24297/jaa.v10i0.8061 Impatiens ‘New Guinea’ (Impatiens hawkeri Bull) Hormonal Effects during the Post-transplant Biomass Accumulation https://cirworld.com/index.php/jaa/article/view/8119 <p>Pot ornamental plant productivity is related to the environmental growth facilities but negatively affected by the pot root restriction syndrome so during nursery as the post-transplant stage. The physiological mechanism involved included both the synthesis and translocation of auxins and cytokinins. However, clear sink-source and dose-response relationships of exogenous plant regulators such as indole acetic acid (AIA) and benzyl amino purine (BAP) and environment on biomass accumulation in most ornamental foliage plants, including New Guinea <em>Impatiens </em>(<em>Impatiens hawkeri</em>) are lacking. The aim of this work was to analyze the effects of an exogenously shoot-applied auxin and a cytokinin, separately or successively, on the post-transplant biomass accumulation of <em>I. hawkeri </em>through the anatomical, morphological and physiological changes observed. Two experiments were performed. The first experiment included the response to only BAP-sprayed plants (0, 5, 50, or 100 mg L<sup>-1</sup>). For the second experiment, rooting cuttings of <em>I. hawkeri </em>were sprayed with different concentrations of IAA (0, 5, 50, or 100 mg L<sup>-1</sup>) followed by different BAP concentrations (0, 5, 50, or 100 mg L<sup>-1</sup>) one week later to run-off at sunset. Results showed that (a) a single BAP or AIA dose increased increase post-transplant biomass accumulation through a higher leaf area expansion and photo assimilate production, (b) as a result of both AIA and BAP spray, the higher NAR the higher post-transplant biomass accumulation, (c) leaf anatomical changes (leaf thickness, intercellular spaces) let a higher carbon dioxide diffusion and fixation with a correlative increase in photo assimilates, (d) a higher root system would be related to a higher cytokinin synthesis. In summary, similarities between responses to either hormone, together with the lack of any IAA - BAP interaction, provide two independent routes for commercial growers to increase the productivity of <em>I. hawkeri </em>ornamental plants by using early foliar sprays.</p> Adalberto Di Benedetto Jorge Molinari Pablo Fujinuma Alberto Pagani Marcela Buyatti Ernesto Giardina ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2019-02-28 2019-02-28 10 1629 1649 10.24297/jaa.v10i0.8119 Jatropha curcas: an overview https://cirworld.com/index.php/jaa/article/view/8145 <p><em>Jatropha curcas L</em>. belongs to family <em>Euphorbiaceae</em>, <em>Jatropha curcas</em> is a valuable multi-purpose crop, historically it was used as medicine for wounds and leaves used as drinks against malaria, jatropha plants used to control soil degradation, alleviate erosion, desertification and increase soil fertility, however, in last decades there is more attention to use jatropha oil for produce biodiesel, &nbsp;<em>Jatropha </em><em>curcas </em>is easily propagated by seeds or stem cutting, it is tolerant for drought for longtime, it is grow well with treated wastewater, also, it can be grown on marginal land. <em>Jatropha curcas</em> seed have about 32-40% valuable oil used to produce biofuel, therefore, it could be the source for biodiesel production particularly in arid and semiarid regions.</p> Waleed Abobatta ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2019-02-28 2019-02-28 10 1650 1656 10.24297/jaa.v10i0.8145 Heavy metals concentrations in bee products collected from contaminated and non-contaminated areas from Upper Egypt Governorates https://cirworld.com/index.php/jaa/article/view/8149 <p>Twenty fresh clover honey, ten beeswax and ten bee bread samples represented&nbsp;contaminated and non-contaminated areas were collected directly from the apiaries&nbsp;during 2015 .The aim of this study was to evaluate the presence of toxic metals ( Lead&nbsp;(Pb), Cadmium (Cd), Iron (Fe), Copper (Cu) and Zinc (Zn)) in honey, beeswax and&nbsp;bee bread stored inside honey bee colonies. The highest lead contents (0.5488 mg/kg)&nbsp;was estimated in honey samples collected from industrialized area The lowest Pb&nbsp;content were estimated in honey samples collected from rural area (0.5096 mg/kg).<br>The lowest Cd concentration (0.0961 mg/kg). However, the highest content of Cd&nbsp;(0.1042 mg/kg) was recorded in honey samples collected from urbanized areas. High&nbsp;concentration of (Cu) was estimated in honey samples collected from apiaries located&nbsp;in industrialized area (0.0757 mg/kg) while the lowest were recorded in rural area&nbsp;(0.0432 mg/kg) . Zn occurred in low concentration in honey samples The highest&nbsp;value was recorded in honey samples from rural area (0.241) mg/kg and the lowest in&nbsp;honey samples from apiaries located in Reclaimed soils (0.185) mg/kg. Heavy metal<br>concentrations of Pb, Cd, Fe, Cu and Zn in beeswax samples collected from&nbsp;contaminated and non-contaminated areas were 1.388, 0.194, 16.696, 0.619 and 4.606&nbsp;mg/kg. While the averages of heavy metal concentrations in non contaminated area&nbsp;decreased to 1.175; 0.160; 15.466; 0.391 and 2.520 mg/kg, respectively.&nbsp;Contamination in bee bread samples showed that lead concentration (1.094 mg/kg to&nbsp;1.338 mg/kg) was detected in bee bread samples collected from honey bee colonies&nbsp;located in non-contaminated areas and samples collected from( industrialized and&nbsp;urban areas).</p> Abd El-Aleem Saad Desoky N. S. Omran M.O. M. Omar M. H. Hussein M. M. Abd-Allah ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2019-02-28 2019-02-28 10 1657 1666 10.24297/jaa.v10i0.8149 Rodent Control Strategies in Hospitals https://cirworld.com/index.php/jaa/article/view/8158 <p>An infestation of rodents in a healthcare facility is especially dangerous because these&nbsp;pests can carry disease and spoil food with the&nbsp;bacteria&nbsp;and&nbsp;viruses&nbsp;they harbor in&nbsp;their saliva and droppings. Exposure to dangerous pathogens could be disastrous for&nbsp;already health-compromised patients.&nbsp;The aim of the study is to implement an integrated control program for rodents inside&nbsp;hospitals and to clarify the most important preventive methods that can be used in the&nbsp;control process and to make some important observations in the application of&nbsp;methods of integrated control of rodents to obtain the best program for rodent control&nbsp;in hospitals.</p> Abd El-Aleem Saad Desoky ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2019-03-28 2019-03-28 10 1667 1668 10.24297/jaa.v10i0.8158 Micropropagation and Conservation of Fig (Ficus Carica L.) https://cirworld.com/index.php/jaa/article/view/8160 <p>An efficient protocol is outlined for rapid and mass micropropagation of <em>Ficus carica</em> L. (fig). Shoot tips (5 mm) were obtained from mother plants stock grown on half strength Murashige and Skoog (½ MS) medium with the addition of 30 g/L sucrose. For shoot multiplication Benzyl amino purine (BAP) and kinetin produced differences number of new shoot per plant and shoot height. BAP at 0.4 mg/L in combination with 0.2 mg/L indole-3-butyric-acid (IBA) produce maximum <em>in vitro </em>propagation rate, with 4.2 shoots per ex-plant. Root initiation was experimented on MS medium containing different concentrations of mg/L, IBA, IAA (Indole-3-acetic-acid) (IAA) or Naphthalene acetic acid (NAA). Highest number of root (4.3) was resulted when 1.5 mg/L IBA was used. After acclimatization in a mixture of (1 soil: 1 perlite: 1 peat) survival rate of 80% was achieved. For <em>in vitro</em> conservation of <em>F. carica</em> was experimented as microshoots were stored for 40 weeks on MS medium containing different sucrose concentration. Medium supplemented with 3% sucrose gave the highest regrowth (89%) at 24 ± 2 °C. Culture grew slowly when transferred to new fresh medium after the storage periods.</p> Mohamad Shatnawi Rida A. Shibli Wesam G. Shahrour Tamara S. Al-Qudah Abu-Zahra Taleb ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2019-03-28 2019-03-28 10 1669 1679 10.24297/jaa.v10i0.8160 Soil Physical Properties Enhancement Via Native Tree Species in Northern Ethiopia https://cirworld.com/index.php/jaa/article/view/8203 <p>Dozens of chemical fertilizer is produced in factors to maintain and reclaim soil fertility, but the reliance on artificial fertilizer alone is not advisable due to environmental pollution. . Thus, indigenous plant species can maintain soil fertility without any extra cost. The aim of this study was to examine the effect of indigenous tree species on soil physical properties. Three dominant indigenous trees species (Croton macrostachuys, Cordia Africana and Albizia gummifera) were considered.&nbsp; Soil samples were taken from different sampling points under crown of these tree species (mid of crown and edge of crown). One sampling point is included by far 30m from the selected tree as a control group. Soil sample was taken by using core sampler. Soil physical properties such as texture, structure, porosity, bulk density and moisture content were analyzed. Bulk density was determined as 0.73g/cm<sup>3</sup>, 0.75g/cm<sup>3</sup> and 0.8g/cm<sup>3</sup> for Albizzia gummifera, Croton macrostachuys and Cordia Africana respectively. Bulk density was very small under crown of all trees as influenced by the amount of organic matter falling from trees leaf. The texture under crown of all tree species (silty loam, loam and loamy sand) is quite better for agriculture purpose than control point. Soil color under crown is in the range of brown to black color, which indicates high fertility level. Soil porosity was very high under the crown of all tree species as compared to the control site. It is determined as 72.5%, 71.6%, and 69.7% for Albizzia gummifera, Croton macrostachuys and Cordia Africana respectively. Soil consistence, porosity and moisture content were better under crown of the trees than control group. Soil properties under the crown of indigenous tree species were better than bare land soils.&nbsp; Therefore, indigenous trees are promising option to maintain soil fertility level and land owners ought to be aware of this miracle.</p> Kassaye Gurebiyaw Legese Abay Gelanew Melese Alemu ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2019-03-29 2019-03-29 10 1680 1691 10.24297/jaa.v10i0.8203 Effect of Micronutrients on The Two Qatari Date Palm Cultivars Shishi and Lulu Through in Vitro Technic https://cirworld.com/index.php/jaa/article/view/8252 <p>Plant tissue responds to nutrient media due to the plant cultivars genetic diversity. The objective of this research is to determine the effects of micronutrients on date palm growth and which of these micronutrients are critical for improving growth combined with Paclobutrazol or without on <em>in vitro</em> of elongation stage. <em>In vitro</em> growth of two cultivars were determined by varying five treatments that included minor salts (EDTA-chelated iron, CuSo<sub>4,</sub>) alone or combined with Paclobutrazol. The effects of these five treatments on plantlet length, trunk thickness, rooting number and Chlorophyll A, B was investigated. The results obtained after three months showed that, increasing copper sulphate on MS elongation media combined with Paclobutrazol was more effective for increasing the trunk thickness as well as rooting number compared with normal MS level. While poor response was found when Fe was increased in the chelated form of Fe-EDTA on MS elongation media.&nbsp; For cultivars, Shishi gave the highest plantlets length as well as root number. The maximum content of chlorophylls A and B were observed when copper sulphate was increased on MS elongation media with cultivar Lulu. The survival rate after 3 months was related to the medium used during the elongation phase, the survival of plantlets reached to 98.66% with cultivar lulu at the acclimatization stage.</p> Sara Aqeel Rehab A. Sidky ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2019-01-31 2019-01-31 10 1692 1704 10.24297/jaa.v10i0.8252 Inoculation of Potato Pulp with Antibacterial Lactic Acid Bacterium To Improve The Quality of Livestock Feed https://cirworld.com/index.php/jaa/article/view/8199 <p>Potato pulp was inoculated either with the antibacterial lactic acid bacteria <em>Lactococcus lactis</em> and <em>L</em><em>.</em> <em>diolivorans</em> and the inoculated potato pulp was ensiled under anaerobic conditions for 30 d at room temperature in a mini-silo. We have previously reported that <em>L</em>. <em>diolivorans</em> produces antimicrobial peptides with potent antibacterial activity; therefore, the bacterium is expected to increase the fermentation quality of the potato pulp. The quality of the potato pulp silage was evaluated. The moisture content of the potato pulp silage was remained 822 g/kg before and after ensiling. The protein content in the silage increased from an initial concentration of 39 to 57 g/kg and 58 g/kg for <em>L. lactis</em> and <em>L</em><em>. </em><em>diolivorans</em> inoculations, respectively. The lactic acid content significantly increased from 2 to 52 g/kg (<em>L. lactis</em>) and 50 g/kg (<em>L. </em><em>diolivorans</em>) after ensiling, whereas, toxic butyric acid was not detected with either treatment. These results suggest that the inoculation of potato pulp with <em>L. lactis</em> or <em>L. </em><em>diolivorans</em> increases the quality and nutrition of potato pulp as silage. In particular,<em> L</em>. <em>diolivorans</em> is an efficient inoculant because it produces antibacterial peptides that prevent the increase of saprophyte in silage.</p> Ayiguli Dagaerbieke Oyundelger Ganzorig Kensuke Miyazaki Takashi Yoshida ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2019-04-05 2019-04-05 10 1705 1712 10.24297/jaa.v10i0.8199