The Stimulating Effects of Imazalil and Carbendazim Fungicides on in Vitro Propagation and ex Situ conservation of the Medicinal Balkan Range-Restricted Sideritis Raeseri Boiss & Heldr. Subsp. Raeseri

  • Virginia Sarropoulou Hellenic Agricultural Organization (HAO) – DEMETER
  • Eleni Maloupa Hellenic Agricultural Organization (HAO) – DEMETER
Keywords: Benzymidazoles, Carbendazim, Fungicides, Mountain Tea of Parnassus or Velouchi, Imazalil, Imidazoles, Micropropagation, Sideritis Raeseri Boiss. & Heldr. Subsp, Raeseri, Vitrification

Abstract

Sideritis raeseri Boiss & Heldr. subsp. raeseri, known in Greece as Mountain tea of Parnassus or Velouchi is a range restricted medicinal plant of the Balkan peninsula. Conventional propagation methods did not allow the mass production of plant material in a short period of time due to both low seed germination rate and rooting of cuttings. Therefore, the aim of this study was to establish a reliable, reproducible and efficient regeneration protocol for mass and large-scale micropropagation, germplasm and ex situ conservation of S. raeseri Boiss & Heldr. using Imazalil and Carbendazim fungicides. After 9 weeks, 2.5-10 mg/l Imazalil stimulated root length by 1 cm but diminished root number and rooting percentage. Optimum shoot number (5.58), shoot length (24.91 mm), shoot multiplication (100%), root number (20.63) and rooting (66.67%) were recorded with 0.5 mg/l kinetin (KN) + 0.05 mg/l ?-napthaleneacetic acid (NAA) (Imazalil-free). After 4 ½ weeks, 1 mg/l Carbendazim + 0.5 mg/l 6-benzyladenine (BA) yielded 5.77 shoots/explant, 16.1 mm shoot length and 100% shoot multiplication. However, Carbendazim did not result in rooting. The ex vitro survival percentage of rooted shoot-tip explants derived from Imazalil experiment was 95%. Carbendazim proved useful in shoot proliferarion and Imazalil in root elongation of S. raeseri Boiss & Heldr. subsp. raeseri micropropagation system.

 

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Author Biographies

Virginia Sarropoulou, Hellenic Agricultural Organization (HAO) – DEMETER

Hellenic Agricultural Organization (HAO) – DEMETER, Institute of Plant Breeding and Genetic Resources, Laboratory of Protection and Evaluation of Native and Floricultural Species, Balkan Botanic Garden of Kroussia, P.C. 570 01 Thermi, Thessaloniki, P.O. Box 60458, Greece

Eleni Maloupa, Hellenic Agricultural Organization (HAO) – DEMETER

Hellenic Agricultural Organization (HAO) – DEMETER, Institute of Plant Breeding and Genetic Resources, Laboratory of Protection and Evaluation of Native and Floricultural Species, Balkan Botanic Garden of Kroussia, P.C. 570 01 Thermi, Thessaloniki, P.O. Box 60458, Greece

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Published
2019-05-30
How to Cite
Sarropoulou, V., & Maloupa, E. (2019). The Stimulating Effects of Imazalil and Carbendazim Fungicides on in Vitro Propagation and ex Situ conservation of the Medicinal Balkan Range-Restricted Sideritis Raeseri Boiss & Heldr. Subsp. Raeseri. JOURNAL OF ADVANCES IN AGRICULTURE, 10, 1752-1763. https://doi.org/10.24297/jaa.v10i0.8282
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Articles