Unique Localization of Bovine Viral Diarrhea Virus Non-Structural NS4B Protein in Infected Cells

  • Yuto Suda Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657
  • Daisuke Yamane Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657
  • Muhammad Atif Zahoor Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657
  • Yassir Mahgoub Mohamed Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657
  • Shin Murakami Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657
  • Kentaro Kato National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555
  • Taisuke Horimoto Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657
  • Hiroomi Akashi Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657
Keywords: Bovine viral diarrhea virus, Non-structural protein, Localization

Abstract

Bovine viral diarrhea virus (BVDV), an important pathogen infecting ruminants, has 2 biotypes: cytopathic (cp) and noncytopathic (ncp), which are related to the onset of disease. The viral replication complex is composed of viral non-structural (NS) proteins, raising the possibility that NS protein(s) play a role in viral biotypes. To gain insight into this possible role, we analysed the intracellular localization of each NS protein in both cp and ncp virus-infected cells, and found that NS4B protein, a possible anchor protein of the viral replication complex, showed a unique dotted localization pattern that markedly merged with an endoplasmic reticulum marker, unlike other NS proteins, although there was no difference in the localization of NS4B protein between the 2 biotypes. 

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Author Biographies

Yuto Suda, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657
Department of Veterinary Microbiology
Daisuke Yamane, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657
Department of Veterinary Microbiology
Muhammad Atif Zahoor, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657
Department of Veterinary Microbiology
Yassir Mahgoub Mohamed, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657
Department of Veterinary Microbiology
Taisuke Horimoto, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657
Department of Veterinary Microbiology
Hiroomi Akashi, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657
Department of Veterinary Microbiology
Published
2016-07-20
How to Cite
Suda, Y., Yamane, D., Atif Zahoor, M., Mohamed, Y., Murakami, S., Kato, K., Horimoto, T., & Akashi, H. (2016). Unique Localization of Bovine Viral Diarrhea Virus Non-Structural NS4B Protein in Infected Cells. JOURNAL OF ADVANCES IN AGRICULTURE, 6(1), 914-921. https://doi.org/10.24297/jaa.v6i1.5396
Section
Articles