Cloning, expression, and enzymatic activity analysis of strawberry S-adenosyl-L-metnionine synthetase gene FaSAMS1

  • Shu-Fang Wang College of Plant Science and Technology, Beijing University of Agriculture, No.7 Beinong Road, Changping District, Beijing 102206, People’s Republic of China
  • Li-Na Hua College of Plant Science and Technology, Beijing University of Agriculture, No.7 Beinong Road, Changping District, Beijing 102206, People’s Republic of China
  • Xiaofeng Liu College of Plant Science and Technology, Beijing University of Agriculture, No.7 Beinong Road, Changping District, Beijing 102206, People’s Republic of China
  • Yuanyue Shen College of Plant Science and Technology, Beijing University of Agriculture, No.7 Beinong Road, Changping District, Beijing 102206, People’s Republic of China
Keywords: Strawberry, fruit ripening, S-adenosyl-L-methionine synthetase (SAMS), yeast expression

Abstract

The S-adenosyl methionine synthetase gene FaSAMS1 is involved in the regulation of strawberry fruit ripening, however, the biochemical properties of FaSAMS1 protein remain unclear. Here, a coding cDNA sequence of FaSAMS1 was cloned by RT-PCR and inserted into a recombinant yeast expression vector pPICZA, then transformed into the yeast expression strain X-33. The fusion protein FaSAMS1 was induced, expressed, and purified. The enzymatic activity analysis of FaSAMS1 showed that the reaction system contains 0.09 mg of FaSAMS1, protein concentration reached at 0.454 mg·mL-1, the activity of the enzyme was 0.32 U, specific activity was 3.56 U·mg-1. This study has provided biochemical evidence for the involvement of ethylene in the regulation of strawberry fruit ripening. 

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Author Biographies

Shu-Fang Wang, College of Plant Science and Technology, Beijing University of Agriculture, No.7 Beinong Road, Changping District, Beijing 102206, People’s Republic of China
Beijing Key Laboratory of New Technology in Agricultural Application
Li-Na Hua, College of Plant Science and Technology, Beijing University of Agriculture, No.7 Beinong Road, Changping District, Beijing 102206, People’s Republic of China
Beijing Key Laboratory of New Technology in Agricultural Application
Xiaofeng Liu, College of Plant Science and Technology, Beijing University of Agriculture, No.7 Beinong Road, Changping District, Beijing 102206, People’s Republic of China
Beijing Key Laboratory of New Technology in Agricultural Application
Yuanyue Shen, College of Plant Science and Technology, Beijing University of Agriculture, No.7 Beinong Road, Changping District, Beijing 102206, People’s Republic of China
Beijing Key Laboratory of New Technology in Agricultural Application
Published
2016-04-30
How to Cite
Wang, S.-F., Hua, L.-N., Liu, X., & Shen, Y. (2016). Cloning, expression, and enzymatic activity analysis of strawberry S-adenosyl-L-metnionine synthetase gene FaSAMS1. JOURNAL OF ADVANCES IN AGRICULTURE, 6(1), 841-845. https://doi.org/10.24297/jaa.v6i1.5390
Section
Articles