Extraction and Purification of Antimicrobial Proteins from Datura Stramonium Seed
Resistant to antibiotics by microbes have become a major global challenge incurring economic and public health burden. Hence, research to develop new effective anti-biotics should be a major consideration to pharmaceutical industries, scientific researchers, Amanda ‘scientific researchers and world health organizations at large. This study is conducted to investigate the antimicrobial property of proteins present in the seeds of Datura stramonium. The extraction was carried out using Tris-HCL buffer prepared from 50M Tris and 0.3M NaCL, the proteins were isolated using ammonium sulphate precipitation to obtain 80% fraction. The isolated and extracted proteins sample were subjected to dialysis in which all the salt was removed and then purified using Ion-exchange chromatography. Acidic and basic fractions of the proteins obtained were subjected to SDS-PAGE electrophoresis to visualize their different molecular weight. More protein band was observed in the basic fraction between 9-45kDa. Antibacterial activities of both acidic and basic proteins were carried out using the paper disc diffusion method against clinical bacterial isolates of E. coli and Klebsiella pneumoniae. More activity was observed in basic protein with a diameter 8mm compared to the acidic fraction of 7mm in diameter.
R. E. W. Hancock and A. Patrzykat, “Clinical Development of Cationic Antimicrobial Peptides?: From Natural to Novel Antibiotics,” no. 604, pp. 79–83, 2002.
P. Soni, A. A. Siddiqui, J. Dwivedi, and V. Soni, “Pharmacological properties of Datura stramonium L . as a potential medicinal tree?: An overview,” vol. 2, no. 12, pp. 1002–1008, 2012.
R. Singh and C. Suresh, “Purification and Characterization of a Small Chito-specific Lectin from Datura innoxia Seeds Possessing Anti-microbial Properties,” Int. J. Biochem. Res. Rev., vol. 9, no. 2, pp. 1–17, 2016.
A. S. I. Y. P, M. M. P, A. M. P, and F. Garc, “Novel defensin subfamily from spinach ( Spinacia oleracea ),” vol. 435, pp. 159–162, 1998.
E. P. Rodrigues, A. R. Torres, J. S. da Silva Batista, L. Huergo, and M. Hungria, “A simple, economical and reproducible protein extraction protocol for proteomics studies of soybean roots,” Genet. Mol. Biol., vol. 35, no. SUPPL.1, pp. 348–352, 2012.
K. Thevissen, A. Ghazi, G. W. De Samblanx, C. Brownlee, R. W. Osborn, and W. F. Broekaert, “Fungal Membrane Responses Induced by Plant Defensins and Thionins *,” vol. 271, no. 25, pp. 15018–15025, 1996.
S. Dixit, S. K. Upadhyay, H. Singh, and B. Pandey, “Pectin Methylesterase of Datura species , purification , and characterization from Datura stramonium and its application,” no. February 2017, 2013.
C. Gurramkonda et al., “recombinant protein production with Pichia pastoris?: Application to intracellular production of Hepatitis B surface antigen,” vol. 8, pp. 1–8, 2009.
D. Water and D. Water, “Data Data Social Social,” pp. 199–231, 2013.
Copyright (c) 2019 Surayya Mustapha Muhammad, Ibrahim A. Sabo, Ahmed Mohammed Gumel, Fatima I, Alkali
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors retain the copyright of their manuscripts, and all Open Access articles are distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.