Virulence and Antimicrobial Characteristics of Escherichia Coli Isolated from Diseased Chickens in China and Algeria

  • Junwei Wang China
  • Ge Zhao Ministry of Agriculture and Rural Affairs, China
  • Yubin Gao Qingdao Agricultural University
  • Hefei Xu 4Qingdao International Travel Healthcare Center, China
  • Lounis Mohamed Université Ziane Achour
  • Jianmei Zhao China Animal Health and Epidemiology Center
  • Wenyan Gai China Animal Health and Epidemiology Center
  • Ming Zou Qingdao Agricultural University
  • Zhigang Cui Chinese Center for Disease Control and Prevention, China
  • Shigan Yan Qilu University of Technology
  • Juan Wang Laboratory of Pathogenic Microorganism Inspection, China
  • Zhina Qu Laboratory of Pathogenic Microorganism Inspection, China
Keywords: Pathogenic characteristic, Antimicrobial resistance, Virulence gene, Genotyping, Poultry E. coli

Abstract

To reveal and compare the prevalence of pathotypes and virulence genes, as well as antimicrobial resistance and genotyping of poultry E. coli isolates from China and Algeria. Pathotype and seven virulence genes were tested by PCR, susceptibility to antimicrobials was evaluated using broth microdilution method, and genotyping was analyzed by PFGE. Six isolates were identified as pathogenic E. coli. Virulence gene testing showed that the frequency of ompT, iss, fimC, iroN, hlyF and iutA was high in the isolates from Shandong, Shanxi, Jiangsu and Xinjiang province, while stx2 was detected in only two isolates from Shandong, and stx2, iss, fimC, hlyF and iutA could not be detected in Tibet isolates. Importantly, nearly all isolates from Algeria carried seven virulence genes. Drug resistance testing of 141 strains showed that 98.2% (109/111) of the isolates from China and all isolates (30/30) from Algeria resist to more than three classes of antimicrobials. The PFGE genotyping of 157 isolates yielded 134 types, demonstrated a high level of genetic diversity among these isolates. Thus, the poultry E. coli from both China and Algeria exhibited either high frequencies of antimicrobial resistance or high rates of virulence genes carrying.

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Author Biographies

Junwei Wang, China

Laboratory of Pathogenic Microorganism Inspection, China Animal Health and Epidemiology Center, Qingdao
266032, China;

Ge Zhao, Ministry of Agriculture and Rural Affairs, China

Laboratory of Pathogenic Microorganism Inspection, China Animal Health and Epidemiology Center, Qingdao
266032, China

Yubin Gao, Qingdao Agricultural University

College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China

Hefei Xu, 4Qingdao International Travel Healthcare Center, China

Qingdao International Travel Healthcare Center, Qingdao 266071, China

Lounis Mohamed, Université Ziane Achour

Faculté des Sciences de la Nature et de la Vie, Université Ziane Achour, BP 3117, Route de Moudjbara, 17000

Jianmei Zhao, China Animal Health and Epidemiology Center

Laboratory of Pathogenic Microorganism Inspection, China Animal Health and Epidemiology Center, Qingdao 266032, China

Wenyan Gai, China Animal Health and Epidemiology Center

Laboratory of Pathogenic Microorganism Inspection, China Animal Health and Epidemiology Center, Qingdao 266032, China

Ming Zou, Qingdao Agricultural University

College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China

Zhigang Cui, Chinese Center for Disease Control and Prevention, China

Chinese Center for Disease Control and Prevention, Beijing 102206, China

Shigan Yan, Qilu University of Technology

School of Bioengineering, Qilu University of Technology, Jinan 250353, China

Juan Wang, Laboratory of Pathogenic Microorganism Inspection, China

Laboratory of Pathogenic Microorganism Inspection, China Animal Health and Epidemiology Center, Qingdao 266032, China

Zhina Qu, Laboratory of Pathogenic Microorganism Inspection, China

Laboratory of Pathogenic Microorganism Inspection, China Animal Health and Epidemiology Center, Qingdao
266032, China;

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Published
2019-09-20
How to Cite
Wang, J., Zhao, G., Gao, Y., Xu, H., Mohamed, L., Zhao, J., Gai, W., Zou, M., Cui, Z., Yan, S., Wang, J., & Qu, Z. (2019). Virulence and Antimicrobial Characteristics of Escherichia Coli Isolated from Diseased Chickens in China and Algeria. JOURNAL OF ADVANCES IN AGRICULTURE, 10, 1821-1833. https://doi.org/10.24297/jaa.v10i0.8425
Section
Articles