Micropropagation and Conservation of Fig (Ficus Carica L.)

  • Mohamad Shatnawi Al-Balqa' Applied University
  • Rida A. Shibli University of Jordan
  • Wesam G. Shahrour Al-Balqa' Applied University
  • Tamara S. Al-Qudah University of Jordan
  • Abu-Zahra Taleb Al-Balqa' Applied University
Keywords: Conservation, Germplasm, Ficus Carica, Micropropagation

Abstract

An efficient protocol is outlined for rapid and mass micropropagation of Ficus carica L. (fig). Shoot tips (5 mm) were obtained from mother plants stock grown on half strength Murashige and Skoog (½ MS) medium with the addition of 30 g/L sucrose. For shoot multiplication Benzyl amino purine (BAP) and kinetin produced differences number of new shoot per plant and shoot height. BAP at 0.4 mg/L in combination with 0.2 mg/L indole-3-butyric-acid (IBA) produce maximum in vitro propagation rate, with 4.2 shoots per ex-plant. Root initiation was experimented on MS medium containing different concentrations of mg/L, IBA, IAA (Indole-3-acetic-acid) (IAA) or Naphthalene acetic acid (NAA). Highest number of root (4.3) was resulted when 1.5 mg/L IBA was used. After acclimatization in a mixture of (1 soil: 1 perlite: 1 peat) survival rate of 80% was achieved. For in vitro conservation of F. carica was experimented as microshoots were stored for 40 weeks on MS medium containing different sucrose concentration. Medium supplemented with 3% sucrose gave the highest regrowth (89%) at 24 ± 2 °C. Culture grew slowly when transferred to new fresh medium after the storage periods.

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Author Biographies

Mohamad Shatnawi, Al-Balqa' Applied University

Biotechnology Department. Faculty of Agricultural Technology. Al-Balqa' Applied University, Al-Salt 19117, Jordan.

Rida A. Shibli, University of Jordan

Department of Horticulture and Agronomy, Faculty of Agriculture. University of Jordan, Amman, Jordan.

Wesam G. Shahrour, Al-Balqa' Applied University

Biotechnology Department. Faculty of Agricultural Technology. Al-Balqa' Applied University, Al-Salt 19117, Jordan

Tamara S. Al-Qudah, University of Jordan

Hamdi Mango Center for Scientific Research University of Jordan, Amman, Jordan *

Abu-Zahra Taleb, Al-Balqa' Applied University

Biotechnology Department. Faculty of Agricultural Technology. Al-Balqa' Applied University, Al-Salt 19117, Jordan

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Published
2019-03-28
How to Cite
Shatnawi, M., Shibli, R. A., Shahrour, W. G., Al-Qudah, T. S., & Taleb, A.-Z. (2019). Micropropagation and Conservation of Fig (Ficus Carica L.). JOURNAL OF ADVANCES IN AGRICULTURE, 10, 1669-1679. https://doi.org/10.24297/jaa.v10i0.8160
Section
Articles